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Knowledge Base

How do I change the deuterium lamp on a Dionex UltiMate 3000 Series HPLC system?

LD-DIO-105LLIf the lamp on your Dionex UltiMate 3000 Series HPLC system fails, follow the instructions below to replace your Deuterium lamp.
  1. Turn off the power to the detector. The deuterium lamp emits UV radiation that is harmful to the eyes when switched on.
  2. Allow the lamp and lamp housing to cool.
  3. Open the front panel on the detector.
  4. You will see two knurled head screws on the flow cell cover and a further two screws on the tubing bracket, remove these four screws and then remove the cover and bracket.
  5. You will find that the flow cell inlet tubing is pushed into the tubing guide in the lamp cover, remove this.
  6. To remove the flow cell, squeeze the handle and pull to release it from the optical bench.
  7. Carefully move the flow cell tubing out of the way and remove the four screws on the lamp cover.
  8. Remove the outer cover and set it aside.
  9. You will see two crosshead screws that hold the metal connector in place on the right, remove these and the cover.
  10. To remove the lamp, squeeze the clip on the connector and disconnect from the 6-pin bulkhead connector that is on the side wall.
  11. After loosening the three captive screws in the lamp flange the lamp should simply pull out of the optical bench.
  12. Remove the new lamp from it's packaging and check it for fingerprints and dust. If necessary you can clean the lamp with isopropyl alcohol.
  13. To insert the new lamp first line up the lamp flange with the two locating pins and three threaded holes in the optical bench.
  14. When the flange is aligned, gently push the lamp into place. As opposed to many other optical detectors, the PDA-3000 deuterium lamp forward light beam points away from the flow cell when installed correctly.
  15. To ensure proper performance, the lamp must be fully seated. When the lamp is fully seated, tighten the captive screws in the flange.
  16. Squeeze the clip on the UV lamp connector and insert it back into the 6-pin bulkhead connector on the side wall.
  17. Replace the lamp cover and reroute the wires through the guides on the lamp cover inside taking care not to pinch the UV lamp connector wires under the lamp cover.
  18. To replace the flow cell, squeeze the handle and insert it into the optical bench. Release the handle and pull the flow cell forward slightly until it clicks. If the flow cell does not click into place at first, try rotating the handle slightly.
  19. Thread the flow cell inlet tubing through the tubing guide on the lamp cover and reinstall the tubing bracket.
  20. Thread the flow cell outlet tubing through the slot on the side of the flow cell cover and replace the cover. Tighten the two screws on the cover fingertight. Take care not to pinch the tubing under the flow cell cover.
  21. Close the front door on the detector.
  22. Turn the detector power back on and start the Chromeleon server.
  23. Reset the lamp lifetime by pressing F8 to display the 'Commands' dialog box.
  24. Select 'UV'.
  25. Select the 'UVLampAge' command.
  26. Reset the number to zero and click 'Execute'.
  27. Run the wavelength calibration & wavelength verification by navigating to the folder 'Dionex Templates/Panels/Dionex_LC'
  28. Open the 'UltiMate_3000_PDA-3000.pan' control panel.
  29. Click the 'Diagnostics' button. The PDA-3000 diagnostics panel opens.
  30. Click the 'wavelength' script button under 'Calibration'. The detector will perform the wavelength calibration routine and upload the result (pass or fail) to Chromeleon.
  31. Repeat steps 27 - 29 but this time click the 'wavelength verification' script button under 'Diagnostic tests'. The detector will perform the wavelength verification test and upload the result (pass or fail) to Chromeleon.
  32. If the either test fails, call the Kinesis servicing team for support.
  33. Allow the new lamp to run in for a minimum of 24 hours before running your first analysis. During this period, strong baseline fluctuations and increased noise may occur.
  Order your replacement lamp Kinesis have been manufacturing lamps for over 10 years now and with sales of over 10,000 lamps per year, we are the largest 3rd party lamp manufacturer in the world. This means that Kinesis are able to offer the highest quality lamps with large price saving benefits. The benefits of Kinesis lamps include:
  • Large Price Savings over branded spares with no reduction in quality
  • Bare lamps sourced from the same factories as used by the original instrument manufacturers
  • All guarantees and warranties match those offered by the OEM
  • ISO certified and industry verified manufacturing processes
  • Lamps individually QC tested at every processing step
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