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TELOS MicroPlate™: Extract Small Volume Biological Fluid Samples with Confidence

Ordering Information Request a Sample & Claim Voucher Download the Brochure

25% off your first TELOS MicroPlate order

  • Versatile 96-well format for small volume biological fluids
  • Optimised well geometry for 5 and 10mg sorbent mass
  • 50µl elution volumes for minimal sample dilution and reduced evaporation times
  • Populated plates and loose wells
  • Compatible with vacuum and positive pressure
  • Automation compatible
Introduction

Developments in LC-MS/MS detection levels have seen biological fluid sample volumes reduced to an extent that conventional SPE formats are not always suitable, or provide considerable analyte dilution and extended evaporation times.

When extracting from sample volumes of less than 100µl, it is important the elution volumes and the sample preparation format are fully compatible with the original sample volumes being processed.

The TELOS MicroPlate™ is optimised for the sample processing and extraction of small volume biological fluids. The internal diameter, frit design and sorbent allow biological fluids to be processed. The TELOS MicroPlate is a modular design, allowing for flexibility in sample numbers. Full or partially populated plates can be processed using vacuum or positive pressure. In addition, the well outlet design ensures good collection plate penetration, removing any possibility of well to well cross contamination.

Sorbents

The TELOS MicroPlate is available in 5 and 10mg sorbent masses, packed with five TELOS neo SPE Sorbents - PRP, PCX, WCX, PAX and WAX.  

Well Design

The individual wells of the TELOS MicroPlate are designed to both allow maximum interaction between the analyte and sorbent and ensure elution volumes are kept to a minimum. This is particularly important for sample volumes of 100µl and less, and ensures minimal evaporation times prior to analysis by LC-MS.

Elution Volumes and Recoveries

The optimised well and frit design (see Figure 1) of the TELOS MicroPlate provide an overall bed volume of 10 - 15µl. A well packed with 5mg sorbent allows analytes to be eluted in as little as 50µl.

Analyte

Concentration

Mean Recovery (%)

RSD (%)

Metoprolol

0.5ng/ml

86.8

3.93

2.5ng/ml

92.6

3.12

10ng/ml

93.9

2.21

Propranolol

1ng/ml

88.1

3.54

5ng/ml

90.2

2.03

020ng/ml

95.6

3.17

Populated Plates and Loose Wells

The TELOS MicroPlate is available as either populated plates or loose wells. This allows the product to be used for both high throughput assays (populated plate) and assays where the sample numbers vary, populating the base plate with the required number of wells. When using vacuum, the unrequired ports on the base plate can be sealed. The individual wells can be removed or added very easily, with a secure “click” when the wells are added to the base plate.

Vacuum and Positive Pressure

The TELOS MicroPlate is compatible with both vacuum and positive pressure manifolds. The well outlets extend beyond the plate “shelf” and ensure they fully penetrate the collection plate.

Recovery & Elution Volumes

The TELOS MicroPlate 5mg plate allows elution volumes of 50µl. The following recovery data and elution volumes are for two ß-blockers, Metoprolol and Propranolol, when extracted from human plasma (50µl) using a TELOS neo PRP 5mg MicroPlate.  


Ordering Information

The TELOS MicroPlate is available as either populated plates or loose wells.

TELOS MicroPlate: Populated Plates:
Part Number Description Pack Size Buy Online
TELOS neo PRP
600-005M-096MP TELOS neo PRP MicroPlate, 5mg (populated plate) Each Add to Basket
600-010M-096MP TELOS neo PRP MicroPlate, 10mg (populated plate) Each Add to Basket
TELOS neo PCX
620-005M-096MP TELOS neo PCX MicroPlate, 5mg (populated plate) Each Add to Basket
620-010M-096MP TELOS neo PCX MicroPlate, 10mg (populated plate) Each Add to Basket
TELOS neo WCX
640-005M-096MP TELOS neo WCX MicroPlate, 5mg (populated plate) Each Add to Basket
640-010M-096MP TELOS neo WCX MicroPlate, 10mg (populated plate) Each Add to Basket
TELOS neo PAX
660-005M-096MP TELOS neo PAX MicroPlate, 5mg (populated plate) Each Add to Basket
660-010M-096MP TELOS neo PAX MicroPlate, 10mg (populated plate) Each Add to Basket
TELOS neo WAX
680-005M-096MP TELOS neo WAX MicroPlate, 5mg (populated plate) Each Add to Basket
680-010M-096MP TELOS neo WAX MicroPlate, 10mg (populated plate) Each Add to Basket
TELOS MicroPlate: Loose Wells
Part Number Description Pack Size Buy Online
TELOS neo PRP
600-005M-096LW TELOS neo PRP MicroPlate, 5mg (loose wells) 100 Add to Basket
600-010M-096LW TELOS neo PRP MicroPlate, 10mg (loose wells) 100 Add to Basket
TELOS neo PCX
620-005M-096LW TELOS neo PCX MicroPlate, 5mg (loose wells) 100 Add to Basket
620-010M-096LW TELOS neo PCX MicroPlate, 10mg (loose wells) 100 Add to Basket
TELOS neo WCX
640-005M-096LW TELOS neo WCX MicroPlate, 5mg (loose wells) 100 Add to Basket
640-010M-096LW TELOS neo WCX MicroPlate, 10mg (loose wells) 100 Add to Basket
TELOS neo PAX
660-005M-096LW TELOS neo PAX MicroPlate, 5mg (loose wells) 100 Add to Basket
660-010M-096LW TELOS neo PAX MicroPlate, 10mg (loose wells) 100 Add to Basket
TELOS neo WAX
680-005M-096LW TELOS neo WAX MicroPlate, 5mg (loose wells) 100 Add to Basket
680-010M-096LW TELOS neo WAX MicroPlate, 10mg (loose wells) 100 Add to Basket
TELOS MicroPlate: Method Development Kit:
Part Number Description Pack Size Buy Online
000-010M-096LW TELOS MicroPlate Method Development Kit, 10mg (loose wells)* 100 Add to Basket

*contains 20 x 10mg wells of TELOS neo PRP, PCX, WCX, PAX and WAX, a base plate and base plate sealing strips

TELOS MicroPlate: Accessories:
Part Number Description Pack Size Buy Online
000-0000-096BP TELOS MicroPlate Base Plate 5 Add to Basket
000-0000-096SS TELOS MicroPlate Base Plate Sealing Strips 25 Add to Basket

Request your evaluation plate and receive 25% off your first order*

* Offer only applies to customers who have not previously purchased TELOS MicroPlate products from Kinesis. Offer applies to your first order of TELOS MicroPlate products only. Offer expires March 31st 2017.


Download a Brochure

Click a thumbnail below to download the TELOS Microplate brochure or a copy of our scientific poster.

    TELOS MicroPlate Brochure

    3.45 MB

    Date posted: 25th Nov 2013 , 5:11pm